rf value of aspirin

. Transcribed image text: TABLE 1: Rf VALUES OF KNOWN ANALGESİCS IN ETHYL ACETATE/HEXANE SOLVENT Acetaminophen Aspirin Caffeine Naproxen о. Rf is retention factor, it is defined as the distance travelled by the compound divided by the distance travelled by the solvent. Retardation Factor (Rf) • Experimental data show that aspirin is the least polar among the three, followed by acetaminophen, then caffeine, the most polar. Synthesis of Acetylsalicylic Acid (Aspirin) Anna Shahrour Partner Jocelyn Barraza TA Yong Wang Section 05 Introduction Aspirin is the common name for the compound acetylsalicylic acid, widely used as a fever reducer and as a painkiller. Therefore, the Rf value was calculated base on the formula below. First, an average molecular weight of 1.7 x 10 2 g/mol was derived from titrations (table 2.2). distinguishing compounds with similar Rf values. What is the Rf value for aspirin? Created Date: 20070122133001Z . Two pigments with the same Rf value are likely to be identical molecules. It is a modifier of the enzymatic activity of cyclooxygenase-2 (COX-2). I've been trawling the web but I can't find anything on the Rf values of aspirin in 2:1 Hexane: Ethyl Acetate and also in 65:30:5 Hexane: Ethyl Acetate: Acetic Acid. Small Rf values tend to indicate larger, less soluble pigments while the highly soluble pigments have an Rf value near to one. There was no spot for fraction 11 indicating that the tube contained . 81 TABLE 2: ANALYSIS OF UNKNOWN LIQUID IN ETHYL ACETATE/HEXANE SOLVENT Unknown # Identity of Compound Rf Naproxen Spot 1 Spot 2* Spot 3* Acetaminopnen NAA NIA * Unknown may contain as few as one or as many as three compounds 2. And for my Rf values I got… Rf (reference - salicylic acid and ethyl acetate) = 2.47 ÷ 5.30 = 0.466 . It also blocks thromboxane A2 on platelets in an irreversible fashion preventing platelet aggregation. This is all in relation to a purity test of aspirin compared to salicylic acid. This is all in relation to a purity test of aspirin compared to salicylic acid. Identify the components of the mixture, and identify each of the Rf = 0.78 (Ethanol-Methanol mixture {1:2}) - a mixture of 1 part . What are the units for Rf values? Because mixture solvents are often applied Rf values are usually written as the following examples: Rf = 0.66 (60% Ethanol) - if % is given it is assumed that the mixture is in water hence 60% ethanol 40% water. Similarly, what is the RF value of aspirin? TLC was done on a regular silica plate. The Rf value for paracetamol Rf = 3.90/6.1 Rf value = 0.639 = 0.64 RESULTS (b). Unlike other NSAIDs (ibuprofen/naproxen), which bind reversibly to this enzyme, aspirin binding is irreversible. It is calculated by dividing the distance moved by the solvent by the distance moved by the spot. as explained above. Below is a group TLC of all 20 aminoacids plus some other related compounds. I've been trawling the web but I can't find anything on the Rf values of aspirin in 2:1 Hexane: Ethyl Acetate and also in 65:30:5 Hexane: Ethyl Acetate: Acetic Acid. Analysis Of Rf Value Of Caffeine. That is incredibly specific. Rf values do not have units since it is a ration of distances. The hardiness of the method is evidenced by the Rf values, which vary by no more than 3% over a four-month period. For ibuprofen, the Rf value is 1.32/1.48 equals 0.89, and for acetaminophen, the Rf value is 1.07/1.48 equals 0.72. Rf Values The Rf value for a substance is the distance that substance travels compared to the distance the solvent travels. light or iodine vapour. In the example below, the Rf value for substance A is equal to x/a. Rf Values The Rf value for a substance is the distance that substance travels compared to the distance the solvent travels. (r) Fractions 1, 3, 4, and 5 did not show most likely because of poor spotting and should have been re-spotted for better analysis. Rf Values Name Distance from Base to Center of Spot (cm) ÷ Distance from Base to Sovlent Front (cm) = Rf Value acetaminophen 4.0 ÷ 5.5 = .73 aspirin 4.7 ÷ 5.5 = .85 caffeine 2.4 ÷ 5.5 = .44 salicylamide 4.6 ÷ 5.5 = .84 Anacin 2.6 ÷ 5.8 = .45 Bufferin Read More Essay about Isolation of Components of Bc Powder 1655 Words | 7 Pages ! 7) whereas the RF value of paracetamol (RF 0.62) decreases. Rf = 0.78 (Ethanol-Methanol mixture {1:2}) - a mixture of 1 part . ; So, an aspirin solution is to be run against a polar solvent (mobile phase) when the stationary phase is less polar than the mobile one. Solid aspirin waste can go in the trash can. "Aspirin Synthesis - Alternate (Non-Mi ro a e) Pro edure" pg. Rf value = distance travelled by substance divided by the distance travelled by solvent. Trace the TLC plates and associated spots into your laboratory notebook, and note any distinguishing features. Other names: Benzoic acid, 2-(acetyloxy)-; Salicylic acid acetate; o-Acetoxybenzoic acid; o-Carboxyphenyl acetate; A.S.A. Substances: Aspirin Caffeine Acetaminophen IbuprofenExcedrin Anacin Rf Values: 0.45 0.08 0.24 0.60 0.09 0.10 0.26 0.46 0.39 Aspirin Caffeine Ibuprofen Acetaminophen Exc. . Finally, the Rf values of unknown samples were compared with Rf values of the standard samples. When the second TLC showed . Aspirin Rf. Analyze Polarity vs Rf Value. Calculate the R. f values for each spot. . It seems as if the acetaminophen and ibuprofen readings were off. Retention factors range between zero and one. Rf value = distance travelled by substance divided by the distance travelled by solvent. National Library of Medicine. For it to be a valid comparison, the TLC plates must be run under the exact same conditions for temperature, stationary phase, and mobile phase. That being said, aspirin travelled the greatest distance and has the highest Rf value. Procedure. Thin-Layer Chromatography. This experiment uses TLC to analyse standards of caffeine and three analgesics, acetylsalicylic acid (aspirin), acetaminophen (paracetamol) and ibuprofen. The Rf values are 0.70 for salicylic acid and diflunisal, 0.67 for aspirin and methyl salicylate, 0.60 for gentisic acid, 0.57 for p-aminosalicyclic acid, and 0.40 for salicyluric acid. Background. (l), An. Linearity ranges, mean recoveries and relative standard deviations in calibration graphs of the proposed method were . Mechanism of Action. Acetylsalicylic acid is expected to have a lower Rf value than salicylic acid. For both of these known drugs, the Rf values acquired were close to my predictions before the experiment. fof the presence of impure aspirin in the product, but indistinctly hinted at the remaining presence of unacetylated salicylic acid, enabling the conclusion of an uncompleted reaction process. To confirm the results for the known drugs, we can find the literature value of the Rf. 1+2.) value of 0.50, indicating that the component was aspirin. 3.4 Hazardous Waste and Clean-up 3.4.1 Hazardous Waste Pour the used TLC solvent into the waste container in the hood at the front of the lab. Similar Rf values will not necessarily mean that the two components are the same molecule. The Rf values are 0.70 for salicylic acid and diflunisal, 0.67 for aspirin and methyl salicylate, 0.60 for gentisic acid, 0.57 for p-aminosalicyclic acid, and 0.40 for salicyluric acid. Since the chromatogram did not develop, there was no way to observe the distance the dot or the solvent traveled, therefore not supplying an Rf value. Thin-layer chromatography (TLC) is an important technique in organic chemistry. Between aspirin and acetaminophen, which would travel through the HPLC the slowest and have the . Again, if you look in the literature for TLC results of aspirin and salicylic acid, you will find varying Rf values, so one shouldn't be overly concerned about it. Theoretical Yield of Aspirin (g)= 5.4 Mass of Crude Aspirin (g)= 2.6 % yield of Crude Aspirin= 48 % Mass of Recrystallized Aspirin (g)= 1.7 % yield of Recrystallized Aspirin= 31% 1.Using your yield of recrystallized product, determine the mass ofsalicyclic acid needed make 100 tablets of extra strength aspirinif each tablet has 500 mg of . The Rf values are always different for different stationary and mobile phases used. Half-fill a 50 cm 3 beaker with deionised water, and heat to 70-80 °C. All three samples seem similar which suggests our class samples have the same purity as the manufactured aspirin. The less polar the sample, the less it binds to the plate and the faster it travels, increasing the Rf value. The hardiness of the method is evidenced by the Rf values, which vary by no more than 3% over a four-month period. There was a misreading of the TLC plates which resulted in a miscalculation of the Rf values. Compound B had an rf value of 0.77 which is . Rf values do not have units since it is a ration of distances. Aspirin is a cyclooxygenase-1 (COX-1) inhibitor. The Rf values indicate how soluble the particular pigment is in the solvent by how high the pigment moves on the paper. PubChem . Separations and spot shapes are . TLC can also be used to identify compounds by comparison with known samples, check the relative . For fractions 6-10, the R. f. value was 0.63 indicating that the component was aspirin. Different analytes can be distinguished by their Rf values as well as by their appearance under UV light or after treatment with other visualising reagents. Because aspirin is polar (I believe would be considered polar because it dissolves in water despite some other sources saying that it is actually non-polar) and the silica in the TLC plates are also polar, aspirin would not be able to travel very far because it would interact with the silica. TLC is a type of adsorption chromatography, and the most common substrates used for the stationary phases in the lab, are silica (SiO 2) and alumina (Al 2 O 3).It is recommended that you read the page on adsorption chromatography before doing this experiment. The retention factor (rf) of a particular material is the ratio of the distance the spot moved above the origin to the distance the solvent front moved above the origin. IUPAC Standard InChIKey: BSYNRYMUTXBXSQ-UHFFFAOYSA-N Copy CAS Registry Number: 50-78-2 Chemical structure: This structure is also available as a 2d Mol file or as a computed 3d SD file The 3d structure may be viewed using Java or Javascript. Cinnamedrine is actually a mixture of 3 parts aspirin to 1 part caffeine, and is in both Anacin and Vanquish c) Cinnamedrine is . RF value of aspirin (RF 0.32) and ascorbic acid (RF 0.14) (Fig. In general, low polarity compounds have higher R f values than higher polarity compounds. Because mixture solvents are often applied Rf values are usually written as the following examples: Rf = 0.66 (60% Ethanol) - if % is given it is assumed that the mixture is in water hence 60% ethanol 40% water. In order to determine whether an unknown compound is identical to a compound of known structure, it is necessary to run the two samples side by side on the same TLC plate, preferably at the same concentration. 81 TABLE 2: ANALYSIS OF UNKNOWN LIQUID IN ETHYL ACETATE/HEXANE SOLVENT Unknown # Identity of Compound Rf Naproxen Spot 1 Spot 2* Spot 3* Acetaminopnen NAA NIA * Unknown may contain as few as one or as many as three compounds 2. If the solvent system was changed, which solvent system would result in a DECREASE in the Rf value of aspirin (solvent structures shown beside the TLC plate)? If a permanent record is needed, take a photo as the spots of colour will fade. The Rf value for the Ibuprofen was ~0.56, ~0.47 for acetaminophen, ~0.16 for caffeine, and ~2.7 for aspirin. Detection limits of 1 ppm or less for all the analytes compared favorably to limits of more than 20 ppm for Trinder's test. 149 O. There are several things to be taken away from the collected data in regards to the purity of the synthesized aspirin sample. [20] (CI 7.3) Nuclear Magnetic Resonance (n.m.r . 3.4.2 Clean-up Dry your glassware and return it to your station. The product was analyzed using the melt point, the FeCl3 . TLC uses the different affinities a compound has for the mobile and stationary phases to achieve separation of mixtures of organic compounds. The Rf values are 0.70 for salicylic acid and diflunisal, 0.67 for aspirin and methyl salicylate, 0.60 for gentisic acid, 0.57 for p-aminosalicyclic acid, and 0.40 for salicyluric acid. Rf Values 1 2 3 solvent front baseline s s & p p s starting point p product. Rf stands for retardation factor. This value, compared with the molecular weight of commercial aspirin, C 9 H 8 O 4, will demonstrate the difference in purity.The molecular weight of commercial aspirin is 180 . Finally, the Rf values of unknown samples were compared with Rf values of the standard samples. 24 o. Transcribed image text: TABLE 1: Rf VALUES OF KNOWN ANALGESİCS IN ETHYL ACETATE/HEXANE SOLVENT Acetaminophen Aspirin Caffeine Naproxen о. This temperature range of initial to final melting point has a small and sharp temperature range of only 3˚C, which is within the acceptable limits of the 128-137˚C1 literature value for Aspirin (Acetylsalicylic Acid/2-acetoxybenzoic acid), if located slightly toward the beginning of the . Add 25 drops of ethanoic anhydride followed by one drop of 85% phosphoric acid. Weigh 0.23 g of 2-hydroxybenzoic acid (salicylic acid) into a test tube. For the unknown powder, we obtained Rf values of 0.52 and 0.76 so we believe that the unknown powder contains Aspirin and Ibuprofen. [20] (CI 7.3) Nuclear Magnetic Resonance (n.m.r . Aspirin | C9H8O4 | CID 2244 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more. In the example below, the Rf value for substance A is equal to x/a. Aspirin is a relatively simple molecule containing an ethylated phenol group and a carboxylic acid group. This alteration in RF value of these drugs in the presence of surfactant in solvent systems, may be due to variable hydrophobic or hydrophilic interactions with these drugs. Thin Layer Chromatography Report List the calculated Rf values for aspirin, acetaminophen, ibuprofen, and caffeine in your final eluent system. Rf of Caffeine 0 cm / 2 cm =. Laboratory Manual for Organic Chemistry. The Rf value for aspirin is 1.25/1.48 equals 0.84. 24 o. It is calculated by dividing the distance moved by the solvent by the distance moved by the spot. The NPR includes the admission and discharge dates and the diagnoses at discharge for all hospital stays in Sweden. These values will vary with temperature, humidity, and . values and literature values do not always agree. The International Classification of Diseases 10th Revision (I63 and I64) codes were used for recording cerebral infarction. 1uL of aminoacid solution in water (5mg/mL; 3mg Na2CO3 added for Cystine, Phenylalanine and Tyrosine) was applied (about 3mm spot was formed).This means each spot contained 5ug (65 nmols to 25 nmols) of an aminoacid. The NPR has been validated, and a diagnosis of stroke had a positive predictive value of approximately 90% . Furthermore, the retention factors change depending on the conditions (choice of mobile phase, stationary phase, temperature, etc). Thin layer chromatography (TLC) is used routinely in the laboratory to both monitor reactions and analyse the purity of samples. To confirm the results for the known drugs, we can find the literature value of the Rf. The melting point of the experimentally synthesized Aspirin product was found to be between 126-129 ˚C. d. SUMMARY AND DISCUSSION: After the slides were placed into the jar containing TLC, the chromatogram did not develop for Ibuprofen or Aspirin. Unknown 2 was determined to have aspirin, acetaminophen and caffeine present due to the closeness of Rf value of the known components compared to the Rf values of

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